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Cyclin H (Phospho-Thr315) Antibody#11689

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Product Detail

Product NameCyclin H (Phospho-Thr315) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 315 (E-W-T(p)-D-D) derived from Human Cyclin H .

Target NameCyclin H

ConjugateUnconjugated

Other Namescyclin H; p34; p37; MO15-associated protein;

Accession NoSwiss-Prot#: P51946;
NCBI Gene#: 902;
NCBI Protein#: NP_001230.1.

Uniprot P51946

Gene ID 902;

Sdspage MW36kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody #11689. The lane on the left is treated with antigen-specific peptide.

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Maekelae T.P., Nature 371:254-257(1994).
Fisher R.P., Cell 78:713-724(1994).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

If you have published an article using product 11689, please notify us so that we can cite your literature.

et al,CyclIns B1, T1, and H differ In their molecular mode of Interaction with cytomegalovirus proteIn kInase pUL97. In J Biol Chem on 2019 Apr 12 by SteIngruber M, Keller L, et al.. PMID:30782840 , (2019),
PMID: 30782840

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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