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PAK1 (Phospho-Ser204) Antibody#11748

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Product Detail

Product NamePAK1 (Phospho-Ser204) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous levels of PAK1 only when phosphorylated at serine 204.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 204(T-R-S(p)-V-I) derived from Human PAK1.

Target NamePAK1

ConjugateUnconjugated

Other NamesADRB2; PAK 1; P65-PAK; P68-PAK;

Accession NoSwiss-Prot#: Q13153;
NCBI Gene#: 5058;
NCBI Protein#: NP_002567.3.

Uniprot Q13153

Gene ID 5058;

Sdspage MW65kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 3T3 cells treated with UV using PAK1 (Phospho-Ser204) Antibody #11748.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PAK1 (Phospho-Ser204) antibody #11748 (left)or the same antibody preincubated with blocking peptide (right).

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Brown J.L.,Curr. Biol. 6:598-605(1996).
Sells M.A.,Curr. Biol. 7:202-210(1997).
The MGC Project Team;Genome Res. 14:2121-2127(2004).

If you have published an article using product 11748, please notify us so that we can cite your literature.

et al,PAK1 Is Involved in the Spindle Assembly during the First Meiotic Division in Porcine OocytesInInt J Mol SciOn2023 Jan 6byLei Peng?1,?Yijing He et al..PMID: 36674642 , (2023),
PMID: 36674642
et al,PAK1-Dependent Regulation of Microtubule Organization and Spindle Migration Is Essential for the Metaphase I–Metaphase II Transition in Porcine Oocytes In Biomolecules on 2024 Feb 17 by Lei Peng, Yijing He,et al..PMID:38397472 , (2024),
PMID: 38397472

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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