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TIE2 (Phospho-Tyr992) Antibody#11756

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Product Detail

Product NameTIE2 (Phospho-Tyr992) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 992 (E-V-Y(p)-V-K) derived from Human TIE2.

Target NameTIE2

ConjugateUnconjugated

Other NamesAngiopoietin-1 receptor,EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2;

Accession NoSwiss-Prot#: Q02763;
NCBI Gene#: 7010;
NCBI Protein#: NP_000450.2.

Uniprot Q02763

Gene ID 7010;

Sdspage MW126kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody #11756.The lane on the right is treated with the antigen-specific peptide.

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Schooley K.A., Baum P.R. Oncogene 8:663-670(1993)
Dunham I. Nature 429:369-374(2004)
Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

If you have published an article using product 11756, please notify us so that we can cite your literature.

et al,Tie2 signalling through Erk1/2 regulates TLR4 driven inflammation.In Cell Signal. On 2018 Nov by Smith TL, Van Slyke P et al..PMID: 30077653 , (2018),
PMID: 30077653

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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