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Smad2 (Phospho-Ser250) Antibody#11924

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Product Detail

Product NameSmad2 (Phospho-Ser250) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Smad2 only when phosphorylated at serine 250.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine250(E-L-S(p)-P-T) derived from Human Smad2 .

Target NameSmad2

ConjugateUnconjugated

Other NamesJV18-1; MADH2; Mad-related protein 2; Mothers against DPP homolog 2;

Accession NoSwiss-Prot#: Q15796;
NCBI Gene#: 4087;
NCBI Protein#: NP_001003652.1

Uniprot Q15796

Gene ID 4087;

Sdspage MW65kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 cells treated with PMA using Phospho-Smad2 (Ser250) antibody #11924.The lane on the right is treated with the antigen-specific peptide.

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagu¨¦ J (1999)Genes Dev 13, 804-16 .

If you have published an article using product 11924, please notify us so that we can cite your literature.

et al,TGF β1 mediated Smad signaling pathway and EMT in hepatic fibrosis induced by Nano NiO in vivo and in vitro.In Environ Toxicol on 2020 Apr by Zhang Q, Chang X ,et al..PMID: 31737983 , (2020),
PMID: 31737983

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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