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CDC25B (Phospho-Ser353) Antibody#11949

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Product Detail

Product NameCDC25B (Phospho-Ser353) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of CDC25B only when phosphorylated at serine 353.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 353(R-R-S(p)-V-T) derived from Human CDC25B .

Target NameCDC25B

ConjugateUnconjugated

Other NamesCDC25M2; MPIP2; Dual specificity phosphatase Cdc25B; CDC25HU2;

Accession NoSwiss-Prot#: P30305;
NCBI Gene#: 994;
NCBI Protein#: NP_001274445.1

Uniprot P30305

Gene ID 994;

Sdspage MW64kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Western blotting: 1:500~1:1000
Western blot analysis of extracts from COS7 cells treated with Etoposide using Phospho-CDC25B (Ser353) antibody #11949.The lane on the right is treated with the antigen-specific peptide.

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Reboutier D, et al. (2012) J Cell Biol 197, 19-26
Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90
Feng C, et al. (2007) Biol Reprod 77, 560-8

If you have published an article using product 11949, please notify us so that we can cite your literature.

et al,Aurora kinase A is essential for meiosis in mouse oocytes. In PLoS Genet on 2021 Apr 26 by Cecilia S Blengini, Patricia Ibrahimian,et al..PMID:33901174 , (2021),
PMID: 33901174
et al,Spatio-temporal requirements of Aurora kinase A in mouse oocytes meiotic spindle building. In iScience on 2024 Jul 3 by Cecilia S Blengini, Michaela Vaskovicova,et al..PMID:39081293 , (2024),
PMID: 39081293

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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