Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > FAK (Phospho-Tyr576) Antibody

FAK (Phospho-Tyr576) Antibody#11957

Availability: Yes
Total Amount:$
Product Detail

Product NameFAK (Phospho-Tyr576) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Tyrosine576 (S-T-Y(p)-Y-K) derived from Human FAK.

Target NameFAK

ConjugateUnconjugated

Other NamesFADK 1; FAK1; Focal adhesion kinase 1; PTK2; pp125FAK

Accession NoSwiss-Prot#: Q05397;
NCBI Gene#: 5747;
NCBI Protein#: NP_001186578.1

Uniprot Q05397

Gene ID 5747;

Sdspage MW125kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Western blotting: 1:500~1:1000
Western blot analysis of extracts from NIH-3T3 tissue using FAK (Phospho-Tyr576) antibody #11957.The lane on the right is treated with the antigen-specific peptide.

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Tong L, et al. (2013)Biochim Biophys Acta 1833, 1304-15. Chen S, et al. (2013)Sci Signal 6, ra40. Tian H, et al. (2012)EMBO J 31, 3885-900.

If you have published an article using product 11957, please notify us so that we can cite your literature.

et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer , (2021),
PMID: 34830928

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;