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Cyclin D1 (Phospho-Thr286) Antibody#11968

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Product Detail

Product NameCyclin D1 (Phospho-Thr286) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 286 (A-C-T(p)-P-T) derived from Human Cyclin D1.

Target NameCyclin D1

ConjugateUnconjugated

Other NamesBCL-1 ; oncogene; CYL-1; PRAD1; PRAD1

Accession NoSwiss-Prot#: P24385;
NCBI Gene#: 595;
NCBI Protein#: NP_444284.1

Uniprot P24385

Gene ID 595;

Sdspage MW33kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details
Western blotting: 1:500~1:1000
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.
Western Blot analysis of rat-musle cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:233

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40
Tagliati F, et al. (2006) Endocrinology 147, 3530-8
Zou Y, et al. (2004) J Biol Chem 279, 27790-8

If you have published an article using product 11968, please notify us so that we can cite your literature.

et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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