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Location： Home > Phospho Antibodies > p70 S6 Kinase (Phospho-Thr389/412) Antibody

p70 S6 Kinase (Phospho-Thr389/412) Antibody#11974

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Product Detail

Product Namep70 S6 Kinase (Phospho-Thr389/412) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at tyrosine 389/tyrosine 412.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine389/412(G-F-T(p)-Y-V) derived from Human p70 S6 Kinase .

Target Namep70 S6 Kinase

ConjugateUnconjugated

Other NamesKS6B1; P70-S6K; RPS6KB1; Ribosomal protein S6 kinase; polypeptide 1

Accession NoSwiss-Prot#: P23443;
NCBI Gene#: 6198;
NCBI Protein#: NP_001258971.1

Uniprot P23443

Gene ID 6198;

Sdspage MW70kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) antibody #11974.The lane on the right is treated with the antigen-specific peptide.
Western blotting analysis using p70 S6 Kinase (Phospho-Thr389/412) Antibody #11974.

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Hong S, et al. (2014) J Biol Chem
Puustinen P, et al. (2014) CJ Cell Biol 204, 713-27
Martineau Y, et al. (2014) Mol Cell Biol 34, 1046-53

If you have published an article using product 11974, please notify us so that we can cite your literature.

et al,5-HT 2 receptor mediates high-fat diet-induced hepatic steatosis and very low density lipoprotein overproduction in rats.In Obes Res Clin Pract. On 2018 Jan - Feb by Li X, Guo K et al..PMID: 27133527 , (2018),
PMID: 27133527
et al,EBV-LMP1 Regulating AKT/mTOR Signaling Pathway and WWOX in Nasopharyngeal Carcinoma.In Int J Clin Exp Pathol on 2017 Aug 1 by Lingyan Qin, Xiaohong Li,et al..PMID: 31966718 , (2017),
PMID: 31966718
et al, miR-223 reverses the resistance of EGFR-TKIs through IGF1R/PI3K/Akt signaling pathway.In Int J Oncol.On 2016 May by J Han, F Zhao et al..PMID: 26936292 , (2016),
PMID: 26936292
et al,Long-term Stress with Hyperglucocorticoidemia-induced Hepatic Steatosis with VLDL Overproduction Is Dependent on both 5-HT2 Receptor and 5-HT Synthesis in Liver.In Int J Biol Sci on 2016 Jan 1 by Jihua Fu , Shaoxin Ma et al..PMID: 26884719 , (2016),
PMID: 26884719
et al,Effective treatment with combination of peripheral 5鈥恏ydroxytryptamine synthetic inhibitor and 5鈥恏ydroxytryptamine 2 receptor antagonist on glucocorticoid鈥恑nduced whole鈥恇ody insulin resistance with hyperglycemia.In J Diabetes Investig on 2016 Nov by Shaoxin Ma , Tao Li et al..PMID:27177506 , (2016),
PMID: 27177506
et al,Rapamycin enhances the anti-cancer effect of dasatinib by suppressing Src/PI3K/mTOR pathway in NSCLC cells. In PLoS One on 2015 Jun 10 by Bin Chen, Xin Xu,et al..PMID:26061184 , (2015),
PMID: 26061184

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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