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Location： Home > Phospho Antibodies > CDK1/CDC2 (Phospho-Thr14) Antibody

CDK1/CDC2 (Phospho-Thr14) Antibody#11997

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Product NameCDK1/CDC2 (Phospho-Thr14) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phospho rylation site of threonine14(E-G-T(p)-Y-G) derived from Human CDK1/CDC2 .

Target NameCDK1/CDC2

ConjugateUnconjugated

Other NamesCDC28; CDC2A; CDK1; MPF; kinase Cdc2

Accession NoSwiss-Prot#: P06493
NCBI Gene#: 983
NCBI mRNA#：NM_001170406.1
NCBI Protein#：NP_001163877.1

Uniprot P06493

Gene ID 983;

Sdspage MW34kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.

Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50
Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57

If you have published an article using product 11997, please notify us so that we can cite your literature.

et al,The Role and Mechanism of CRT0066101 as an Effective Drug for Treatment of Triple-Negative Breast Cancer. In Cell Physiol Biochem on 2019 by Liu Y, Wang Y, et al.. PMID:30845378 , (2019),
PMID: 30845378
et al,Adhesion glycoprotein CD44 functions as an upstream regulator of a network connecting ERK, AKT and Hippo-YAP pathways in cancer progression. Oncotarget on 2015 Feb 20 by Shiyi Yu , Xiuxiu Cai et al..PMID: 25605020 , (2015),
PMID: 25605020

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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