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AML1 (Phospho-Ser276) Antibody#12002

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Product Detail

Product NameAML1 (Phospho-Ser276) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of AML1 only when phosphorylated at Serine 276.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 276 (P-I-S(p)-P-G) derived from Human AML1.

Target NameAML1

ConjugateUnconjugated

Other NamesCBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1

Accession NoSwiss-Prot#: Q01196;
NCBI Gene#: 861;
NCBI Protein#: NP_001001890.1

Uniprot Q01196

Gene ID 861;

Sdspage MW50kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Predicted MW: 50kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from JK cells using AML1 (Phospho-Ser276) Antibody #12002.The lane on the left is treated with the antigen-specific peptide.
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.

If you have published an article using product 12002, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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