Product NameE2F1 (Phospho-Thr433) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of E2F1 only when phosphorylated at Threonine 433.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Threonine 433 (D-L-T(p)-P-L) derived from Human E2F1.

Target NameE2F1

Other NamesE2F-1, PBR3, RBAP-1, RBBP-3

Accession NoSwiss-Prot#: Q01094;
NCBI Gene#: 1869;
NCBI Protein#: NP_005216.1

Uniprot Q01094

Gene ID 1869;

Sdspage MW47kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Predicted MW: 47kd
Western blotting: 1:500~1:1000