Product NameEGFR (Phospho-Tyr1016) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of EGFR only when phosphorylated at Tyrosine 1016.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Tyrosine 1016 (D-E-Y(p)-L-I) derived from Human EGFR.

Target NameEGFR

Other NamesERBB, HER1, mENA, ERBB1, PIG61

Accession NoSwiss-Prot#: P00533;
NCBI Gene#: 1956;
NCBI Protein#: NP_005219.2

Uniprot P00533

Gene ID 1956;

Sdspage MW175kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Predicted MW: 175kd
Western blotting: 1:500~1:1000