Product Detail
                                
                                                                        Product NameKIT (Phospho-Tyr703) Antibody
                                                                                 Host SpeciesRabbit
                                         ClonalityPolyclonal
                                                                                PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
                                        ApplicationsWB
                                        Species ReactivityHu
                                        SpecificityThe antibody detects endogenous level of KIT only when phosphorylated at Tyrosine 703. 
                                        Immunogen TypePeptide-KLH
                                         Immunogen DescPeptide sequence around phosphorylation site of Tyrosine 703
(A-L-Y(p)-K-N) derived from Human KIT.
                                         Target NameKIT 
                                                                                                                         Other NamesPBT,  SCFR,  C-Kit,  CD117
                                        Accession NoSwiss-Prot#: P10721; 
NCBI Gene#: 3815; 
NCBI Protein#: NP_000213.1
                                        Uniprot
                                        P10721
                                        
                                        Gene ID
                                        3815;
                                        
                                                                                Sdspage MW145kd
                                        Concentration1.0mg/ml
                                         FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
                                        StorageStore at -20˚C/1 year
                                                                                                                                                         
                                Application Details
                                Predicted MW: 145kd
Western blotting: 1:500~1:1000
                             
                            
                                                                
                                    
                                    Western blot analysis of extracts from HepG2 cells treated with EGF using KIT (Phospho-Tyr703) Antibody  #12017.The lane on the left is treated with the antigen-specific peptide.
                                 
                                                             
                            
                                Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
                            
                            
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