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MITF (Phospho-Ser180/73) Antibody#12018

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Product Detail

Product NameMITF (Phospho-Ser180/73) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of MITF only when phosphorylated at Serine 180/73.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 180(P-N-S(p)-P-M)/Serine 73(Q-E-R(p)-R-E) derived from Human MITF.

Target NameMITF

ConjugateUnconjugated

Other NamesMI, WS2, CMM8, WS2A, bHLHe32

Accession NoSwiss-Prot#: O75030;
NCBI Gene#: 4286;
NCBI Protein#: XP_005264811.1

Uniprot O75030

Gene ID 4286;

Sdspage MW52kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Predicted MW: 52kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Mouse brain tissue using MITF (Phospho-Ser180/73) Antibody #12018.The lane on the right is treated with the antigen-specific peptide.
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

If you have published an article using product 12018, please notify us so that we can cite your literature.

et al,Dioscin Derived From Solanum Melongena L. "Usukawamarunasu" Attenuates 伪-MSH-Induced Melanogenesis in B16 Murine Melanoma Cells via Downregulation of Phospho-CREB and MITF .In J Food Sci on 2015 Oct by Atsuyoshi Nishina, Kodai Ebina et al..PMID:26352003 , (2015),
PMID: 26352003

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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