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SOX-9 (Phospho-Ser181) Antibody#12025

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Product Detail

Product NameSOX-9 (Phospho-Ser181) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of SOX-9 only when phosphorylated at Serine 181.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 181 (R-K-S(p)-V-K) derived from Human SOX-9.

Target NameSOX-9

ConjugateUnconjugated

Other NamesCMD1, CMPD1, SRA1

Accession NoSwiss-Prot#: P48436;
NCBI Gene#: 6662;
NCBI Protein#: NP_000337.1

Uniprot P48436

Gene ID 6662;

Sdspage MW69kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Predicted MW: 69kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 cells using SOX-9 (Phospho-Ser181) Antibody #12025.The lane on the right is treated with the antigen-specific peptide.
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

If you have published an article using product 12025, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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