Product Detail
Product NameSurvivin (Phospho-Thr117) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of Survivin only when phosphorylated at Threonine 117.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of Threonine 117
(K-E-T(p)-N-N) derived from Human Survivin.
Target NameSurvivin
Other NamesAPI4, EPR-1,
Accession NoSwiss-Prot#: O15392;
NCBI Gene#: 332;
NCBI Protein#: NP_001012270.1
Uniprot
O15392
Gene ID
332;
Sdspage MW16kd
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C/1 year
Application Details
Predicted MW: 16kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells using Survivin (Phospho-Thr117) Antibody #12026.The lane on the left is treated with the antigen-specific peptide.
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
If you have published an article using product 12026, please notify us so that we can cite your literature.
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