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TK (Phospho-Ser13) Antibody#12027

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Product Detail

Product NameTK (Phospho-Ser13) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of TK only when phosphorylated at Serine 13.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 13(P-G-S(p)-P-S) derived from Human TK.

Target NameTK

ConjugateUnconjugated

Other NamesThymidine kinase, cytosolic; TK1

Accession NoSwiss-Prot#: P04183;
NCBI Gene#: 7083;
NCBI Protein#: XP_005257688.1

Uniprot P04183

Gene ID 7083;

Sdspage MW25kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details

Western blotting: 1:500~1:2000
Immunohistochemistry: 1:100~1:300

Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using TK (Phospho-Ser13) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using TK (Phospho-Ser13) Antibody. The picture on the right is blocked with the phospho peptide.

TK is a cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

If you have published an article using product 12027, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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