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Ataxin 1 (Phospho-Ser776) Antibody#12034

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Product Detail

Product NameAtaxin 1 (Phospho-Ser776) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of Ataxin 1 only when phosphorylated at Serine 776.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 776 (R-W-S(p)-A-P) derived from Human Ataxin 1.

Target NameAtaxin 1

ConjugateUnconjugated

Other NamesATX1, D6S504E, SCA1

Accession NoSwiss-Prot#: P54253;
NCBI Gene#: 6310;
NCBI Protein#: NP_000323.2

Uniprot P54253

Gene ID 6310;

Sdspage MW87kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details

Predicted MW: 87kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells using Ataxin 1 (Phospho-Ser776) Antibody #12034.The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 5h, using Ataxin 1 (Phospho-Ser776) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of NIH/3T3 cells, using Ataxin 1 (Phospho-Ser776) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function.

If you have published an article using product 12034, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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