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Keratin 8 (Phospho-Ser432) Antibody#12042

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Product Detail

Product NameKeratin 8 (Phospho-Ser432) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of Keratin 8 only when phosphorylated at Serine 432.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 432 (L-T-S(p)-P-G) derived from Human Keratin 8.

Target NameKeratin 8

Other NamesK8, KO, CK8, CYK8, CK-8

Accession NoSwiss-Prot#: P05787;
NCBI Gene#: 3856;
NCBI Protein#: NP_001243211.1

Uniprot P05787

Gene ID 3856;

Sdspage MW53kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Predicted MW: 53kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from A549 cells using Keratin 8 (Phospho-Ser432) Antibody #12042.The lane on the right is treated with the antigen-specific peptide.
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

If you have published an article using product 12042, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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