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p27 Kip1 (Phospho-Thr198) Antibody#12047

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Product Detail

Product Namep27 Kip1 (Phospho-Thr198) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of p27 Kip1 only when phosphorylated at Threonine 198.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Threonine 198 (R-R-R(p)-Q-T) derived from Human p27 Kip1.

Target Namep27 Kip1

ConjugateUnconjugated

Other NamesKIP1, MEN4, CDKN4, MEN1B, P27KIP1

Accession NoSwiss-Prot#: P46527;
NCBI Gene#: 1027;
NCBI Protein#: NP_004055.1

Uniprot P46527

Gene ID 1027;

Sdspage MW27kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Predicted MW: 27kd
Western blotting: 1:500~1:1000

Western Blot analysis of VEC cells using Phospho-p27 (T198) Polyclonal Antibody
Western blot analysis of lysates from HeLa cells, HepG2 cells and COLO cells, using p27 Kip1 (Phospho-Thr198) Antibody. The lane on the right is blocked with the phospho peptide.

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

If you have published an article using product 12047, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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