Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > Na,K-ATPase alpha1 (Phospho-Ser23) Antibody

Na,K-ATPase alpha1 (Phospho-Ser23) Antibody#12052

Availability: Yes
Total Amount:$
Product Detail

Product NameNa,K-ATPase alpha1 (Phospho-Ser23) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityRt

SpecificityThe antibody detects endogenous level of Na,K-ATPase alpha1 only when phosphorylated at Serine 23.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 23 (K-K-S(p)-K-K) derived from Rat Na,K-ATPase alpha1.

Target NameNa,K-ATPase alpha1

Other NamesNkaa1b

Accession NoSwiss-Prot#: P05023;
NCBI Gene#: 476;
NCBI Protein#: NP_000692.2

Uniprot P05023

Gene ID 476;

Sdspage MW113kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details

Predicted MW: 113kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Rat brain tissue using Na,K-ATPase alpha1(Phospho-Ser23) Antibody #12052.The lane on the left is treated with the antigen-specific peptide.

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

If you have published an article using product 12052, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;