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FOXO4 (Phospho-Thr451) Antibody#12053

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Product Detail

Product NameFOXO4 (Phospho-Thr451) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of FOXO4 only when phosphorylated at Threonine 451.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Threonine 451 (L-G-T(p)-P-V) derived from Human FOXO4.

Target NameFOXO4

ConjugateUnconjugated

Other NamesAFX, AFX1, MLLT7

Accession NoSwiss-Prot#: P98177;
NCBI Gene#: 4303;
NCBI Protein#: NP_001164402.1

Uniprot P98177

Gene ID 4303;

Sdspage MW65kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Predicted MW: 65kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HUVEC cells treated with EGF using FOXO4 (Phospho-Thr451) Antibody #12053.The lane on the left is treated with the antigen-specific peptide.
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

If you have published an article using product 12053, please notify us so that we can cite your literature.

et al,FoxO4 activity is regulated by phosphorylation and the cellular environment during dehydration in the African clawed frog, Xenopus laevis.On Int J Neurosci.On 2018 Dec by Zh o H, Liu Y et al..PMID:29883225 , (2018),
PMID: 29883225
et al,Inhibition of skeletal muscle atrophy during torpor in ground squirrels occurs through downregulation of MyoG and inactivation of Foxo4.In Cryobiology on 2016 Oct by Yichi Zhang , Shannon N Tessier et al..PMID:27593478 , (2016),
PMID: 27593478

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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