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p47 phox (Phospho-Ser359) Antibody#12054

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Product Detail

Product Namep47 phox (Phospho-Ser359) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of p47 phox only when phosphorylated at Serine 359.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 359 (Q-R-S(p)-K-P) derived from Human p47 phox.

Target Namep47 phox

ConjugateUnconjugated

Other NamesNCF1A, NOXO2, p47phox, SH3PXD1A

Accession NoSwiss-Prot#: P14598;
NCBI Gene#: 653361;
NCBI Protein#: NP_000256.4

Uniprot P14598

Gene ID 653361;

Sdspage MW45kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Western Blot analysis of HeLa nocodazole 1ug/ml 18h cells using Phospho-p47-phox (S359) Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

If you have published an article using product 12054, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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