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GFAP (Phospho-Ser38) Antibody#12056

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Product Detail

Product NameGFAP (Phospho-Ser38) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of GFAP only when phosphorylated at Serine 38.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Serine 38 (R-L-S(p)-L-A) derived from Human GFAP.

Target NameGFAP

Other NamesGlial fibrillary acidic protein, astrocyte

Accession NoSwiss-Prot#: P14136;
NCBI Gene#: 2670;
NCBI Protein#: NP_001124491.1

Uniprot P14136

Gene ID 2670;

Sdspage MW50kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
Predicted MW: 50kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells using GFAP (Phospho-Ser38) Antibody #12056.The lane on the left is treated with the antigen-specific peptide.
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

If you have published an article using product 12056, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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