Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > PI3-kinase p85- alpha (Phospho-Tyr607) Rabbit mAb

PI3-kinase p85- alpha (Phospho-Tyr607) Rabbit mAb#12057

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(4) Other
Product Detail

Product NamePI3-kinase p85- alpha (Phospho-Tyr607) Rabbit mAb

Clone No.SR4380

Host SpeciesRabbit

ClonalityMonoclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB;IHC

Species ReactivityHuman;Mouse;Rat

SpecificityThe antibody detects endogenous level of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of Tyrosine 607(D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.

Target NamePI3-kinase p85- alpha

ConjugateUnconjugated

Other Namesp85, AGM7, GRB1, p85-ALPHA, PIK3R1

Accession NoSwiss-Prot#: P27986;
NCBI Gene#: 5295;
NCBI Protein#: NP_001229395.1

Uniprot P27986

Gene ID 5295;

Calculated MW84 kDa

Sdspage MW80 kDa

Concentration0.6mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C/1 year

Application Details
WB: 1:200~1:500
IHC: 1:50~1:300
All lanes : Phospho-PI3 Kinase p85α(Tyr607) Rabbit Polyclonal Antibody at 1/500 dilution
Lane 1 : MCF7 treated with serum starvation whole cell lysates
Lane 2 : MCF7 treated with serum for 5min whole cell lysates
Lane 3 : MCF7 treated with serum for 10min whole cell lysates
Lane 4 : MCF7 treated with serum for 20min whole cell lysates
Lane 5 : MCF7 treated with serum for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 8 seconds
All lanes : Phospho-PI3 Kinase p85α(Tyr607) Rabbit Polyclonal Antibody at 1/500 dilution
Lane 1 : MCF7 whole cell lysates
Lane 2 : MCF7 treated with serum for 30min whole cell lysates
Lane 3 : MCF7 treated with 50ng/ml IL-2 for 15min whole cell lysates
Lane 4 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 6 seconds
All lanes : Phospho-PI3 Kinase p85α(Tyr607) Rabbit Polyclonal Antibody at 1/500 dilution
Lane 1 : 3T3 treated with 100ng/ml EGF for 20min whole cell lysates
Lane 2 : C6 treated with serum for 30min whole cell lysates
Lane 3 : PC12 treated with 100ng/ml EGF for 20min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 6 seconds
Formalin-fixed, paraffin-embedded human breast carcinom tissue stained for Phospho-PI3 Kinase p85α(Tyr607) using 12057 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon carcinom tissue stained for Phospho-PI3 Kinase p85α(Tyr607) using 12057 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat colon tissue stained for Phospho-PI3 Kinase p85α(Tyr607) using 12057 at 1/100 dilution in immunohistochemical analysis.

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

If you have published an article using product 12057, please notify us so that we can cite your literature.

et al,SLC3A2 Is Upregulated in Human Osteosarcoma and Promotes Tumor Growth Through the PI3K/Akt Signaling Pathway.In Oncol Rep on 2017 May by Bin Zhu, Dongdong Cheng,et al..PMID: 28350098 , (2017),
PMID: 28350098
et al,Organic anion transporters and PI3K-AKT-mTOR pathway mediate the synergistic anticancer effect of pemetrexed and rhein. In J Cell Physiol on 2020 Apr by Tianci Bu, Changyuan Wang, et al..PMID:31587272 , (2020),
PMID: 31587272
et al,Grape seed proanthocyanidin extract ameliorates cisplatin-induced testicular apoptosis via PI3K/Akt/mTOR and endoplasmic reticulum stress pathways in rats. In J Food Biochem on 2021 Jun 21 by Xuhong Chang, Minmin Tian,et al..PMID:34152018 , (2021),
PMID: 34152018
et al,Spatial dimension cues derived from fibrous scaffolds trigger mechanical activation to potentiate the paracrine and regenerative functions of MSCs via the FAK-PI3K/AKT axis , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors