Product Detail
Product NameRFA2 (Phospho-Thr21) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of RFA2 only when phosphorylated at Threonine 21.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of Threonine 21
(G-Y-T(p)-Q-S) derived from Human RFA2.
Target NameRFA2
Other NamesREPA2, RPA32
Accession NoSwiss-Prot#: P15927;
NCBI Gene#: 6118;
NCBI Protein#: NP_002937.1
Uniprot
P15927
Gene ID
6118;
Sdspage MW35kd
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C/1 year
Application Details
Predicted MW: 35kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Rat heart using RFA2 (Phospho-Thr21) Antibody #12060.The lane on the right is treated with the antigen-specific peptide.
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Ref.9 Ref.14 Ref.15 Ref.16
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
If you have published an article using product 12060, please notify us so that we can cite your literature.
Yes
