Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > Histone H3(Phospho-Ser10) Rabbit Polyclonal Antibody

Histone H3(Phospho-Ser10) Rabbit Polyclonal Antibody#12084

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(5) Other
Product Detail

Product NameHistone H3(Phospho-Ser10) Rabbit Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Rt Ms

SpecificityThe Histone H3(Phospho Ser10) Rabbit Polyclonal Antibody detects endogenous Histone H3(Phospho Ser10) protein.

Immunogen Typepeptide

Immunogen DescPeptide sequence around phosphorylation site of serine 10(R-K-S(p)-T-G) derived from Human Histone H3.

Target NameHistone H3(Phospho-Ser10)

ConjugateUnconjugated

Other NamesH3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody

Accession NoSwiss-Prot#:P68431

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Sdspage MW15-17kd

Concentration1.0mg/ml

FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1μg/ml, 24 hr; +), using #12084 diluted at 1:1,000.
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

If you have published an article using product 12084, please notify us so that we can cite your literature.

et al,Pseudolaric acid B induces mitotic arrest and apoptosis in both imatinib-sensitive and -resistant chronic myeloid leukaemia cells.In Eur J Pharmacol on 2020 Jun 5; by Jiang L, Wen C, et al..PMID: 32179085 , (2020),
PMID: 32179085
et al,Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation.In Nat Cell Biol on 2015 Sep by Yuhui Jiang, Xu Qian et al..PMID:26237645 , (2015),
PMID: 26237645
et al,Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and-resistant colorectal cancer cells.In Cancer Lett on 2016 Dec 28 by Chuangyu Wen , Junxiong Chen et al..PMID: 27713084 , (2016),
PMID: 27713084
et al,PKM2 regulates chromosome segregation and mitosis progression of tumor cells.In Mol Cell on 2014 Jan 9 by Yuhui Jiang, Xinjian Li et al..PMID: 24316223 , (2014),
PMID: 24316223
et al,Focused screening of mitochondrial metabolism reveals a crucial role for a tumor suppressor Hbp1 in ovarian reserve.In Cell Death Differ on 2016 Oct by Z Dong , M Huang et al..PMID:27206316 , (2016),
PMID: 27206316

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors