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Location: Home > Recombinant Rabbit Monoclonal Antibodies > PI3 Kinase p85 (Phospho-Tyr458)/p55 (Phospho-Tyr199) Rabbit mAb

PI3 Kinase p85 (Phospho-Tyr458)/p55 (Phospho-Tyr199) Rabbit mAb#12157

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Product Detail

Product NamePI3 Kinase p85 (Phospho-Tyr458)/p55 (Phospho-Tyr199) Rabbit mAb

Clone No.SR4571

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeIgG

PurificationProtein A

ApplicationsELISA,WB,IF,FC

Species ReactivityHuman,Mouse,Rat

SpecificityPI3 Kinase p85 (Phospho Tyr458)/p55 (Phospho Tyr199) detects endogenous levels of PI3 kinase p85/p55 only when phosphorylated at Human:Y467/Y199, Mouse:Y467/Y199, Rat:Y467/Y199..The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RLyEE

Immunogen DescThe antibody binds to the following modification sequence (lowercase letters are modification sites):RLyEE

Target NamePIK3R1/PIK3R2/PIK3R3

Other NamesPIK3R1 ; GRB1 ; Phosphatidylinositol 3-kinase regulatory subunit alpha ; PI3-kinase regulatory subunit alpha ; PI3K regulatory subunit alpha ; PtdIns-3-kinase regulatory subunit alpha ; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha ; PI3-kinase subunit p85-alpha ; PtdIns-3-kinase regulatory subunit p85-alpha ; PIK3R2 ; Phosphatidylinositol 3-kinase regulatory subunit beta ; PI3-kinase regulatory subunit beta ; PI3K regulatory subunit beta ; PtdIns-3-kinase regulatory subunit beta

Uniprot P27986;Q92569;O00459

Gene ID 5295;8503;5296

Calculated MW85,55kD

Sdspage MW85,55kD

FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB 1:1000-1:5000
IF 1:200-1:1000
ELISA 1:5000-1:20000
All lanes : PI3 Kinase p85 (Phospho-Tyr458)/p55 (Phospho-Tyr199) Rabbit mAb at 1/1k dilution

Lane 1 : PC-12 whole cell lysates
Lane 2 : 3T3-L1 whole cell lysates
Lane 3 : PC-3 whole cell lysates
Lane 4 : U-14 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 85,55 kDa
Observed band size: 85,55 kDa

Exposure time: 5 seconds
All lanes : PI3 Kinase p85 (Phospho-Tyr458)/p55 (Phospho-Tyr199) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : 3T3 treated with Lambda Protein Phosphatase for 30min whole cell
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 85,55 kDa
Observed band size: 85,55 kDa

Exposure time: 7 seconds
Immunocytochemistry/ Immunofluorescence PI3 Kinase p85 (Phospho-Tyr458 )/p55 (Phospho-Tyr199) antibody (12157)

ICC/IF staining of PI3 Kinase p85 (Phospho-Tyr458 )/p55 (Phospho-Tyr199) in PC-3M cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 12157 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],

If you have published an article using product 12157, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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