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FoxO1 (Phospho-Ser256) Polyclonal Antibody#12198

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Product NameFoxO1 (Phospho-Ser256) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF ELISA

Species ReactivityHu Ms Rt

SpecificityPhospho-FoxO1 (S256) Polyclonal Antibody detects endogenous levels of FoxO1 protein only when phosphorylated at S256.

Immunogen Typepeptide

Immunogen DescSynthesized peptide derived from human FoxO1 around the phosphorylation site of S256.

Target NameFoxO1

ConjugateUnconjugated

Other NamesFOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma

Accession NoSwiss-Prot: Q12778
NCBI Gene ID: 2308

Uniprot Q12778

Gene ID 2308;

Sdspage MW70kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Target Specieshuman

Application Details
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/20000
Not yet tested in other applications
Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody

If you have published an article using product 12198, please notify us so that we can cite your literature.

et al,Tanshinone IIA Attenuates Insulin Like Growth Factor 1 -Induced Cell Proliferation in PC12 Cells through the PI3K/Akt and MEK/ERK Pathways.In Int J Mol Sci. On 2018 Sep 12 by Wang H, Su X et al..PMID: 30213025 , (2018),
PMID: 30213025
et al,Kinsenoside attenuates liver fibro-inflammation by suppressing dendritic cells via the PI3K-AKT-FoxO1 pathway. In Pharmacol Res on 2022 Mar by Ming Xiang, Tingting Liu, et al..PMID: 35066108 , (2022),
PMID: 35066108
et al,“Biqi” Bayberry Extract Promotes Skeletal Muscle Fiber Type Remodeling by Increasing Fast Myofiber Formation via the Akt/FoxO1 Pathway in MiceInFoodsOn2023 JulbyJinjie Li, Conceptualization et al..PMID:37444209 , (2023),
PMID: 37444209
et al,Polystyrene nanoplastics with different functional groups and charges have different impacts on type 2 diabetes.In Part Fibre Toxicol on 2024 Apr 24 by Yunyi Wang, Ke Xu,et al..PMID:38658944 , (2024),
PMID: 38658944
et al,Safflower petal water-extract consumption reduces blood glucose via modulating hepatic gluconeogenesis and gut microbiota , (2024),
PMID:
et al,Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice. , (2025),
PMID: 39806512
et al,GYY4137 protects against type 2 diabetes mellitus-associated myocardial autophagy by suppressing FOXO1 signal pathway. , (2025),
PMID: 39777025

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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