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FoxO3A (Phospho-Ser253) Polyclonal Antibody#12199

Availability: Yes
Total Amount:$
Product Detail

Product NameFoxO3A (Phospho-Ser253) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IHC IF ELISA

Species ReactivityHu Ms Rt

SpecificityPhospho-FoxO3A (S253) Polyclonal Antibody detects endogenous levels of FoxO3A protein only when phosphorylated at S253.

Immunogen Typepeptide

Immunogen DescSynthesized peptide derived from human FoxO3A around the phosphorylation site of S253.

Target NameFoxO3A

ConjugateUnconjugated

Other NamesFOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1

Accession NoSwiss-Prot: O43524
NCBI Gene ID: 2309

Uniprot O43524

Gene ID 2309;

Sdspage MW71kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Target Specieshuman

Application Details
Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/20000
Not yet tested in other applications
Western Blot analysis of HELA cells using Phospho-FoxO3A (S253) Polyclonal Antibody

If you have published an article using product 12199, please notify us so that we can cite your literature.

et al,Tanshinone IIA Attenuates Insulin Like Growth Factor 1 -Induced Cell Proliferation in PC12 Cells through the PI3K/Akt and MEK/ERK Pathways.In Int J Mol Sci. On 2018 Sep 12 by Wang H, Su X et al..PMID: 30213025 , (2018),
PMID: 30213025
et al,Amiodarone-induced retinal neuronal cell apoptosis attenuated by IGF-1 via counter regulation of the PI3k/Akt/FoxO3a pathway.In Mol Neurobiol on 2017 Nov by Rifang Liao, Fengxia Yan,et al..PMID: 27774572 , (2017),
PMID: 27774572
et al,MicroRNA-144-3p protects against chemotherapy-induced apoptosis of ovarian granulosa cells and activation of primordial follicles by targeting MAP3K9InEur J Med ResOn2023 Aug 3byMeng Liu?1,?Bang Xiao et al..PMID:?37537658 , (2023),
PMID: 37537658

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;