Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > Smad2/3 (Phospho-Thr8) Polyclonal Antibody

Smad2/3 (Phospho-Thr8) Polyclonal Antibody#12241

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(2) Other
Product Detail

Product NameSmad2/3 (Phospho-Thr8) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB ELISA

Species ReactivityHu Ms Rt

SpecificityPhospho-Smad2/3 (T8) Polyclonal Antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at T8.

Immunogen DescSynthesized peptide derived from human Smad2/3 around the phosphorylation site of T8.

Target NameSmad2/3

ConjugateUnconjugated

Other NamesSMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD3; MADH3; Mothers against decapentaplegic

Accession NoSwiss-Prot: Q15796/P84022
NCBI Gene ID: 4087/4088

Uniprot Q15796

Gene ID 4087;

Calculated MW48/52kDa

Sdspage MW55-62kDa

Concentration1mg/ml

FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

StorageStore at -20˚C/1 year

Target Specieshuman

Application Details

Western blotting: 1/500 - 1/2000
ELISA: 1/10000
Not yet tested in other applications

All lanes : Smad2/3 (Phospho-Thr8) Polyclonal Antibody at 1/500 dilution Lane 1 : 293T whole cell lysates Lane 2 : 293T treated with 20ng/ml TNFa for 30min whole cell lysates Lane 3 : 3T3 whole cell lysates Lane 4 : 3T3 treated with 20ng/ml TNFa for 30min whole cell lysates Lane 5 :Rat brain lysate Lane 6 :Mouse brain lysate Lane 7 :Rat liver lysate Lane 8 :Mouse liver lysate Lane 9 :Mouse kidney lysate Lysates at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 58-62 kDa Exposure time: 2 seconds

If you have published an article using product 12241, please notify us so that we can cite your literature.

et al,Folic acid prevents methotrexate-induced epithelial-mesenchymal transition via suppression of secreted factors from the human alveolar epithelial cell line A549.In Biochem Biophys Res Commun.On 2018 Feb 26 by Kawami M, Harabayashi R et al..PMID:29448106 , (2018),
PMID: 29448106
et al,Arsenic trioxide Inhibits the differentiation of fibroblasts to myofibroblasts through nuclear factor erythroid 2-like 2 (NFE2L2) proteIn and the Smad2/3 pathway. In J Cell Physiol on 2019 Mar by Zhong L, Hao H, et al..PMID:30317545 , (2019),
PMID: 30317545

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors