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EphB1/2 (Phospho-Tyr594/604) Polyclonal Antibody#12273

Availability: Yes
Total Amount:$
Product Detail

Product NameEphB1/2 (Phospho-Tyr594/604) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB IF ELISA

Species ReactivityHu Ms Rt

SpecificityPhospho-EphB1/2 (Y594/604) Polyclonal Antibody detects endogenous levels of EphB1/2 protein only when phosphorylated at Y594/604.

Immunogen Typepeptide

Immunogen DescSynthesized peptide derived from human EphB1/2 around the phosphorylation site of Y594/604.

Target NameEphB1/2

ConjugateUnconjugated

Other NamesEPHB1; ELK; EPHT2; HEK6; NET; Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2; EPHB2; DRT; EPHT3; EPTH3; ERK;

Accession NoSwiss-Prot: P54762/P29323
NCBI Gene ID: 2047/1969

Uniprot P54762

Gene ID 2047;

Sdspage MW110kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Target Specieshuman

Application Details
Western blotting: 1/500 - 1/2000
Immunofluorescence: 1/200 - 1/1000
ELISA: 1/5000
Not yet tested in other applications
All lanes : EphB1/2 (Phospho-Tyr594/604)Antibody at 1/500 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : A431 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa

Exposure time: 7 seconds
Immunofluorescence analysis of HUVEC cells.The picture on the right is blocked with the phospho peptide.

If you have published an article using product 12273, please notify us so that we can cite your literature.

et al,Penta-1,4-dien-3-one and quinoxaline conjugates as potential anticancer agents via inhibiting EphB3/SRC/AKT axis. , (2025),
PMID: 40021052

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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