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L-plastin (Phospho-Ser5) Antibody#12455

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Product Detail

Product NameL-plastin (Phospho-Ser5) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityL-plastin (Phospho-Ser5) Antibody detects endogenous levels of L-plastin only when phosphorylated at Ser5

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human L-plastin (Phospho-Ser5)

Target NameL-plastin

ConjugateUnconjugated

Other NamesLCP1, CP64, L Plastin, LCP-1, LC64P, Lymphocyte cytosolic protein 1, PLS2, L-PLASTIN, Plastin 2, Plastin-2

Accession NoSwiss-Prot#: P13796
NCBI Gene ID: 3936

Uniprot P13796

Gene ID 3936;

Calculated MW70kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Target Specieshuman

Application Details

Western blotting: 1:1000

Western Blot analysis of lysates of 1. K562 cell and 2. K562 cell treated with 100ng/mL LPS for 30min, using primary antibody at 1:1000 dilution.

If you have published an article using product 12455, please notify us so that we can cite your literature.

et al,LRRK1 regulation of actin assembly in osteoclasts involves serine 5 phosphorylation of L plastin,In J Cell Biochem.On 2018 Dec by Si M, Goodluck H et al..PMID:30136304 , (2018),
PMID: 30136304
et al,Adult Osteosclerotic Metaphyseal Dysplasia With Progressive Osteonecrosis of the Jaws and Abnormal Bone Resorption Pattern Due to a LRRK1 Splice Site Mutation.In J Bone Miner Res.On 2020 Mar 2. by Howaldt A, Hennig AF et al..PMID:32119750 , (2020),
PMID: 32119750

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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