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PDK1 (Phospho-Tyr376) Antibody#12481

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Product Detail

Product NamePDK1 (Phospho-Tyr376) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityPDK1 (Phospho-Tyr376) Antibody detects endogenous levels of PDK1 only when phosphorylated at Tyr376

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human PDK1 (Phospho-Tyr376)

Target NamePDK1

ConjugateUnconjugated

Other NamesPDPK1, HPDK1, PkB kinase like gene 1, PkB-like 1, Pdk-1, PkB kinase, PRO0461

Accession NoSwiss-Prot#: O15530
NCBI Gene ID: 5170

Uniprot O15530

Gene ID 5170;

Calculated MW63kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Target Specieshuman

Application Details

Western blotting: 1:1000

If you have published an article using product 12481, please notify us so that we can cite your literature.

et al,Discoidin Domain Receptor 2 Signaling Regulates Fibroblast Apoptosis through PDK1/Akt.In Am J Respir Cell Mol Biol. On 2018 Sep byJia S, Agarwal M et al..PMID: 29652518 , (2018),
PMID: 29652518
et al,Discoidin domain receptor 2 signaling through PIK3C2α in fibroblasts promotes lung fibrosis. In J Pathol on 2024 Apr by Song Ling, Doyun Kwak,et al..PMID:38332727 , (2024),
PMID: 38332727

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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