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CDK1/CDC2 (Phospho-Thr161) Antibody#12493

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Product Detail

Product NameCDK1/CDC2 (Phospho-Thr161) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityCDK1/CDC2 (Phospho-Thr161) Antibody detects endogenous levels of CDK1/CDC2 only when phosphorylated at Thr161

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human CDK1/CDC2 (Phospho-Thr161)

Target NameCDK1/CDC2

ConjugateUnconjugated

Other NamesCDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

Accession NoSwiss-Prot#: P06493
NCBI Gene ID: 983

Uniprot P06493

Gene ID 983;

Calculated MW34kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Target Specieshuman

Application Details
Western blotting: 1:1000
Western Blot analysis of HeLa cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC2 (Phospho-Thr161) Antibody. The picture on the right is blocked with the phospho peptide.

If you have published an article using product 12493, please notify us so that we can cite your literature.

et al,Pseudolaric acid B induces mitotic arrest and apoptosis in both imatinib-sensitive and -resistant chronic myeloid leukaemia cells.In Eur J Pharmacol on 2020 Jun 5; by Jiang L, Wen C, et al..PMID: 32179085 , (2020),
PMID: 32179085
et al,Growth suppression and mitotic defect induced by JNJ-7706621, an inhibitor of cyclin-dependent kinases and aurora kinases. In Curr Cancer Drug Targets on 2012 Jul by A Matsuhashi, T Ohno, et al..PMID:22463590 , (2012),
PMID: 22463590
et al,Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and-resistant colorectal cancer cells.In Cancer Lett on 2016 Dec 28 by Chuangyu Wen , Junxiong Chen et al..PMID: 27713084 , (2016),
PMID: 27713084

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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