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NDR1/2 (Phospho-Thr444/442) Antibody#12521

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Product Detail

Product NameNDR1/2 (Phospho-Thr444/442) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityNDR1/2 (Phospho-Thr444/442) Antibody detects endogenous levels of NDR1/2 only when phosphorylated at Thr444/442

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human NDR1/2 (Phospho-Thr444/442)

Target NameNDR1/2

ConjugateUnconjugated

Other NamesSTK38, NDR1 protein kinase, Nuclear Dbf2-related kinase 1, NDR1, NDR, Ndr protein kinase, Nuclear Dbf2-related 1, Serine/threonine kinase 38

Accession NoSwiss-Prot#: Q15208/Q9Y2H1
NCBI Gene ID: 11329/23012

Uniprot Q15208

Gene ID 11329;

Calculated MW54kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Target Specieshuman

Application Details

Western blotting: 1:1000

Western Blot analysis of lysates of 1. MCF-7 cell and 2. MCF-7 cell treated with 100ng/mL LPS for 30min, using primary antibody at 1:1000 dilution.

If you have published an article using product 12521, please notify us so that we can cite your literature.

et al,Prevention of calpaIn-dependent degradation of STK38 by MEKK2-mediated phosphorylation. In Sci Rep on 2019 Nov 5 by Enomoto A, Fukasawa T,et al..PMID:31690749 , (2019),
PMID: 31690749
et al,NDR1 increases NOTCH1 signaling activity by impairing Fbw7 mediated NICD degradation to enhance breast cancer stem cell properties.In Mol Med. 2022 May 4 by Ling-Ling Wang, Xiao-Yun Wan, et al..PMID:35508987 , (2022),
PMID: 35508987
et al,Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging In EMBO RepOn2023 Dec 6byMonami Ogura , Tatsuya Kaminishi et al..PMID:37987447 , (2023),
PMID: 37987447

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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