Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > ERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187) Antibody

ERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187) Antibody#12548

Availability: Yes
Total Amount:$
Product Detail

Product NameERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187) Antibody detects endogenous levels of ERK1/2 (Thr202+Tyr204/Thr185+Tyr187) only when phosphorylated at Thr202+Tyr204/Thr185+Tyr187

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human ERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187)

Target NameERK1/2

ConjugateUnconjugated

Other NamesMAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Accession NoSwiss-Prot#: P27361/P28482
NCBI Gene ID: 5595/5594

Uniprot P27361

Gene ID 5595;

Calculated MW42kd,44kd

Concentration1.0mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Target Specieshuman

Application Details

Western blotting: 1:1000

If you have published an article using product 12548, please notify us so that we can cite your literature.

et al,TMEM16A-inhibitor loaded pH-responsive nanoparticles: A novel dual-targeting antitumor therapy for lung adenocarcinoma. In Biochem Pharmacol on 2020 Aug by Shuai Guo, Liang Qiu,et al..PMID:32492446 , (2020),
PMID: 32492446
et al,Inhibition of TMEM16A by Natural Product Silibinin: Potential Lead Compounds for Treatment of Lung Adenocarcinoma. In Front Pharmacol on 2021 Apr 14 by Shuai Guo, Xue Bai, et al..PMID:33935737 , (2021),
PMID: 33935737
et al,TMEM16A, a Homoharringtonine Receptor, as a Potential Endogenic Target for Lung Cancer Treatment. In Int J Mol Sci on 2021 Oct 10 by Shuai Guo, Xue Bai, et al..PMID:34681590 , (2021),
PMID: 34681590
et al,Apatinib inhibits pancreatic cancer growth, migration and invasion through the PI3K/AKT and ERK1/2/MAPK pathways , (2021),
PMID: 35116637

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;