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USF1 (Phospho-Thr153) Antibody#12651

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Product Detail

Product NameUSF1 (Phospho-Thr153) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityUSF1 (Phospho-Thr153) Antibody detects endogenous levels of USF1 only when phosphorylated at Thr153

Immunogen TypePeptide

Immunogen DescA synthesized peptide derived from human USF1 (Phospho-Thr153)

Target NameUSF1

ConjugateUnconjugated

Other NamesUSF1, BHLHb11, FCHL, FCHL1, MLTFI, USF, UEF, HYPLIP1, MLTF, Upstream stimulatory factor 1

Accession NoSwiss-Prot#: P22415
NCBI Gene ID: 7391

Uniprot P22415

Gene ID 7391;

Calculated MW34kd

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Target Specieshuman

Application Details

Western blotting: 1:1000

Western Blot analysis of lysates of 1. Hela cell and 2. Hela cell treated with 100ng/mL LPS for 30min, using primary antibody at 1:1000 dilution.

If you have published an article using product 12651, please notify us so that we can cite your literature.

et al,Phosphorylation of E-box binding USF-1 by PI3K/AKT enhances its transcriptional activation of the WBP2 oncogene in breast cancer cells.In FASEB J. On 2018 Sep 5 by Ramos A, Miow QH et al..PMID: 30183375 , (2018),
PMID: 30183375

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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