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Location： Home > Phospho Antibodies > nrf2(Phospho-S40) Antibody

nrf2(Phospho-S40) Antibody#12811

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Product Detail

Product Namenrf2(Phospho-S40) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

Species ReactivityHu Ms Rt

Specificitynrf2(Phospho-S40) Antibody detects endogenous levels of nrf2 only when phosphorylated at S40

Immunogen TypePeptide

Immunogen DescSynthesized peptide derived from human nrf2 around the phosphorylation site of S40.

Target Namenrf2(Phospho-S40)

ConjugateUnconjugated

Other NamesNFE2L2, NF-E2-related factor 2, NFE2-related factor 2, NRF2

Accession NoSwiss-Prot#: Q16236
NCBI Gene ID: 4780

Uniprot Q16236

Gene ID 4780;

Calculated MW68 kDa

Sdspage MW80 kDa

Concentration1.0mg/mL

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Target Specieshuman

Application Details

Western blotting: 1:1000-3000

All lanes: nrf2(Phospho-S40) Rabbit mAb at 1/1k dilution
Lane 1 : HepG2 whole cell lysates
Lane 2 : HepG2 treated with 100ng/ml EGF for 15min whole cell lysates
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with 100ng/ml EGF for 15min whole cell lysates
Lane 5 : Mouse brain lysates
Lane 6 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 80 kDa
Exposure time: 7 seconds

If you have published an article using product 12811, please notify us so that we can cite your literature.

et al,Cocoa ameliorates renal Injury In Zucker diabetic fatty rats by preventIng oxidative stress, apoptosis and Inactivation of autophagy. In Food Funct on 2019 Dec 11 by ?lvarez-Cilleros D , L pez-Oliva ME ,et al..PMID: 31773121 , (2019),
PMID: 31773121
et al,Supplementation with a Cocoa–Carob Blend, Alone or in Combination with Metformin, Attenuates Diabetic Cardiomyopathy, Cardiac Oxidative Stress and Inflammation in Zucker Diabetic Rats. In Antioxidants (Basel) on 2022 Feb 21 by Esther García-Díez, María Elvira López-Oliva, et al..PMID:35204314 , (2022),
PMID: 35204314
et al,Demethylcalabaxanthone from Garcinia mangostana Exerts Antioxidant Effects through the Activation of the Nrf2 Pathway as Assessed via Molecular Docking and Biological EvaluationInAntioxidants (Basel)On2023 Nov 7bySimona De Vita?1,?Milena Masullo et al..PMID:?38001833 , (2023),
PMID: 38001833
et al,Oxidative stress responses and their alterations in the Nrf2-NMDA receptor pathway in the brain of suicide victimsInJ Physiol PharmacolOn2023 JunbyP Panczyszyn-Trzewik et al..PMID:?37661185 , (2023),
PMID: 37661185
et al,Dietary Supplementation with a Cocoa–Carob Blend Modulates Gut Microbiota and Prevents Intestinal Oxidative Stress and Barrier Dysfunction in Zucker Diabetic Rats InAntioxidants (Basel)On2023 AugbyEsther García-Díez,María Elvira López-Oliva et al..PMID:37627514 , (2023),
PMID: 37627514
et al,Cocoa and Carob Supplementation, Alone or in Combination with Metformin, Protects against Hepatorenal Injury in Zucker Diabetic Fatty Rats. In Nutrients on 2024 Sep 13 by Esther García-Díez, María Elvira López-Oliva,et al..PMID:39339687 , (2024),
PMID: 39339687

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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