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Location： Home > Phospho Antibodies > FAK (Phospho-Ser 722) Antibody

FAK (Phospho-Ser 722) Antibody#13327

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Product Detail

Product NameFAK (Phospho-Ser 722) Antibody

Clone No.4G1

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IP, IF, IHC(P)

Species ReactivityHu, Ms, Rt

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesFADK 1 antibody
FADK antibody
FAK related non kinase polypeptide antibody
FAK1 antibody
FAK1_HUMAN antibody
Focal adhesion kinase 1 antibody
Focal adhesion Kinase antibody
Focal adhesion kinase isoform FAK Del33 antibody
Focal adhesion kinase related nonkinase antibody
FRNK antibody
p125FAK antibody
pp125FAK antibody
PPP1R71 antibody
Protein phosphatase 1 regulatory subunit 71 antibody
Protein tyrosine kinase 2 antibody
Protein-tyrosine kinase 2 antibody
Ptk2 antibody
PTK2 protein tyrosine kinase 2 antibody

Accession NoSwiss-Prot#:Q05397

Uniprot Q05397

Gene ID 5747;

Calculated MW125 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at 4˚C

Application Details

WB: 1:100-1:1,000
IHC: 1:50-1:500

IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

A.Western blot analysis of FAK phosphorylation in non-transfected:(A,D), untreated human FAK transfected:(B,E) and lambda protein phosphatase treated human FAK transfected:(C,F) 293T whole cell lysates. Antibodies tested include p-FAK (A-12):(A,B,C) and FAK (C-903):(D,E,F).
B.Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.

Activation of integrins in the extracellular matrix (ECM) of eukaryotic cells promotes the formation of membrane adhesion complexes, known as focal adhesions, which can include cytoskeletal proteins and protein tyrosine kinases, such as focal adhesion kinase (FAK). Phosphorylation events occurring within focal adhesions influence numerous processes that include mitogenic signaling, cell survival and cell motility. FAK is a non-receptor tyrosine kinase that is ubiquitously expressed and highly conserved between species. FAK is recruited by integrin clusters and variably phosphorylated depending on the effector molecules present in the focal adhesion. Phospho-rylation of FAK Tyr 397 decreases during serum starvation, contact inhibition and cell cycle arrest, all conditions under which activating FAK Tyr 407 phosphorylation increases.

If you have published an article using product 13327, please notify us so that we can cite your literature.

et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer , (2021),
PMID: 34830928

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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