Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > Histone H2A.X(Phospho-Ser139) Rabbit mAb

Histone H2A.X(Phospho-Ser139) Rabbit mAb#13343

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(2) Other
Product Detail

Product NameHistone H2A.X(Phospho-Ser139) Rabbit mAb

Clone No.SR33-09

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser139 of human Histone H2A.X.

ConjugateUnconjugated

Other NamesAW228881 antibody
H2A histone family member X antibody
H2A.FX antibody
H2A.X antibody
H2a/x antibody
H2AFX antibody
H2AX antibody
H2AX histone antibody
H2AX_HUMAN antibody
Hist5.2ax antibody
Histone 2A antibody
Histone 2AX antibody
Histone H2A.X antibody
Histone H2AX antibody
RGD1566119 antibody

Accession NoSwiss-Prot#:P16104

Uniprot P16104

Gene ID 3014;

Calculated MWPredicted band size: 15 kDa

Sdspage MWObserved band size: 15 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : Histone H2A.X(Phospho-Ser139) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with UV for 2 hours whole cell lysates
Lane 3 : 293 whole cell lysates
Lane 4 : 293 treated with UV for 2 hours whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 6 seconds
All lanes : Histone H2A.X(Phospho-Ser139) Rabbit mAb at 1/1k dilution
Lane 1 : C6 whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 5 seconds
Formalin-fixed, paraffin-embedded mouse testis tissue stained for Histone H2A.X (Phospho-S139) using 13343 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat colon tissue stained for Histone H2A.X (Phospho-S139) using 13343 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Histone H2A.X (Phospho-S139) antibody (13343)

ICC/IF staining of Histone H2A.X (Phospho-S139) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13343 at a working dilution of 1/100. The secondary antibody was Alexa Fluor®647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. The H2AFX gene is located in close proximity to the Porphobilinogen deaminase (PBG-D) gene in both mouse and human, and maps to chromosome 9 and 11q23, respectively. H2A.X differs from the other members of the H2A family by the presence of a highly conserved C-terminal motif. It is rapidly phosphorylated in response to ionizing radiation and plays an important role in the recognition and repair of DNA double stranded breaks. The phosphorylated form of H2A.X, designated ?-H2A.X, forms nuclear foci at the heavy chain constant region of cells involved in class switch recombination (CSR), a region-specific DNA reaction that replaces one immunoglobulin heavy chain constant region gene with another. The phosphorylated ?-H2A.X is also thought to initiate subsequent repair factors, including Rad50, Rad51 and BRCA1.

If you have published an article using product 13343, please notify us so that we can cite your literature.

et al,NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus. In Hippocampus on 2021 Sep by Yan-Cong Wang, Pu Liu 1, et al..PMID:33960056 , (2021),
PMID: 33960056
et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors