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Location： Home > Phospho Antibodies > FOXO3a(Phospho-S253) Rabbit mAb

FOXO3a(Phospho-S253) Rabbit mAb#13372

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Product Detail

Product NameFOXO3a(Phospho-S253) Rabbit mAb

Clone No.ST49-01

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser253 of human FOXO3a.

ConjugateUnconjugated

Other NamesAF6q21 antibody
AF6q21 protein antibody
DKFZp781A0677 antibody
FKHR2 antibody
FKHRL 1 antibody
FKHRL1 antibody
FKHRL1P2 antibody
Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
Forkhead box O3 antibody
Forkhead box O3A antibody
Forkhead box protein O3 antibody
Forkhead box protein O3A antibody
Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
Forkhead homolog (rhabdomyosarcoma) like 1 antibody
Forkhead in rhabdomyosarcoma like 1 antibody
Forkhead in rhabdomyosarcoma-like 1 antibody
FOX O3A antibody
FOXO2 antibody
foxo3 antibody
FOXO3_HUMAN antibody
FOXO3A antibody
MGC12739 antibody
MGC31925 antibody

Accession NoSwiss-Prot#:O43524

Uniprot O43524

Gene ID 2309;

Calculated MWPredicted band size: 71 kDa

Sdspage MWObserved band size: 90 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : FOXO3A (Phospho-Ser253) Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : 3T3 treated with Lambda Protein Phosphatase for 30min whole cell
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 90 kDa

Exposure time: 6 seconds
All lanes : FOXO3a(Phospho-S253) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 3 : MCF7 whole cell lysates
Lane 4 : Mouse liver lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 71 kDa
Observed band size: 90 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human pancreas tissue stained for FOXO3a(Phospho-S253) using 13372 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for FOXO3a(Phospho-S253) using 13372 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat pancreas tissue stained for FOXO3a(Phospho-S253) using 13372 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence FOXO3a(Phospho-S253) antibody (13372)

ICC/IF staining of FOXO3a(Phospho-S253) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13372 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
FKHRL1 (for forkhead in rhabdomyosarcoma) is a member of the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at threonine-32 and serine-253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephospho-rylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death.

If you have published an article using product 13372, please notify us so that we can cite your literature.

et al, Overexpression of astrocyte-elevated gene-1 is associated with ovarian cancer development and progression.In Mol Med Rep on 2015 Apr by Bo Zhou , Jue Yang et al..PMID:25483832 , (2015),
PMID: 25483832

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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