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MEK1(Phospho-S218/S222) Rabbit mAb#13373

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Product NameMEK1(Phospho-S218/S222) Rabbit mAb

Clone No.ST0490

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser218 and 222 of human MEK1.

ConjugateUnconjugated

Other NamesDual specificity mitogen activated protein kinase kinase 1 antibody
Dual specificity mitogen-activated protein kinase kinase 1 antibody
ERK activator kinase 1 antibody
MAP kinase kinase 1 antibody
MAP kinase/Erk kinase 1 antibody
MAP2K1 antibody
MAPK/ERK kinase 1 antibody
MAPKK 1 antibody
MAPKK1 antibody
MEK 1 antibody
Mek1 antibody
MEKK1 antibody
Mitogen activated protein kinase kinase 1 antibody
MKK 1 antibody
MKK1 antibody
MP2K1_HUMAN antibody
PRKMK1 antibody
Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
Protein kinase mitogen activated, kinase 1 antibody
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Accession NoSwiss-Prot#:Q02750

Uniprot Q02750

Gene ID 5604;

Calculated MWPredicted band size: 45 kDa

Sdspage MWObserved band size: 45 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : MEK1(Phospho-S218/S222) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 200 nM PMA for 20 minutes whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MEK1(Phospho-S218/S222) using 13373 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence MEK1(Phospho-S218/S222) antibody (13373)

ICC/IF staining of MEK1(Phospho-S218/S222) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13373 (red) at a working dilution of 1/100.CYCS Monoclonal antibody (green) was diluted at 1:200.
Goat Anti Rabbit Alexa Fluor 647 was diluted at 1:1000. Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000.
Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.

If you have published an article using product 13373, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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