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Location： Home > Phospho Antibodies > IKB alpha(Phospho-S32) Rabbit mAb

IKB alpha(Phospho-S32) Rabbit mAb#13376

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Product Detail

Product NameIKB alpha(Phospho-S32) Rabbit mAb

Clone No.ST53-05

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.

ConjugateUnconjugated

Other NamesI kappa B alpha antibody
I-kappa-B-alpha antibody
IkappaBalpha antibody
IkB-alpha antibody
IKBA antibody
IKBA_HUMAN antibody
IKBalpha antibody
MAD 3 antibody
MAD3 antibody
Major histocompatibility complex enhancer-binding protein MAD3 antibody
NF kappa B inhibitor alpha antibody
NF-kappa-B inhibitor alpha antibody
NFKBI antibody
NFKBIA antibody
Nuclear factor of kappa light chain gene enhancer in B cells antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

Accession NoSwiss-Prot#:P25963

Uniprot P25963

Gene ID 4792;

Calculated MWPredicted band size: 36 kDa

Sdspage MWObserved band size: 36 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : IKB alpha(Phospho-S32) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 20ng/mL TNF-α for 10 minutes whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 12 seconds
Immunocytochemistry/ Immunofluorescence IKB alpha(Phospho-S32) antibody (13376)

ICC/IF staining of IKB alpha(Phospho-S32) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13376 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence IKB alpha(Phospho-S32) antibody (13376)

ICC/IF staining of IKB alpha(Phospho-S32) in NIH/3T3 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13376 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.

If you have published an article using product 13376, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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