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Location： Home > Phospho Antibodies > PKA R2 (Phospho-S99) Rabbit mAb

PKA R2 (Phospho-S99) Rabbit mAb#13379

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Product NamePKA R2 (Phospho-S99) Rabbit mAb

Clone No.SC54-04

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt, Pig

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA R2

ConjugateUnconjugated

Other NamescAMP dependent protein kinase regulatory subunit alpha 2 antibody
cAMP dependent protein kinase regulatory subunit RII alpha antibody
cAMP dependent protein kinase type II alpha regulatory chain antibody
cAMP dependent protein kinase type II alpha regulatory subunit antibody
cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
KAP2 antibody
KAP2_HUMAN antibody
MGC3606 antibody
PKR 2 antibody
PKR2 antibody
PRKA R2 antibody
PRKAR 2 antibody
PRKAR2 antibody
PRKAR2A antibody
Protein kinase A RII alpha subunit antibody
Protein kinase cAMP dependent regulatory type II alpha antibody

Accession NoSwiss-Prot#:P13861

Uniprot P13861

Gene ID 5576;

Calculated MWPredicted band size: 46 kDa

Sdspage MWObserved band size: 51 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : PKA R2 (Phospho-Ser99) Rabbit mAb at 1/1k dilution
Lane 1 : Mouse Brain lysates
Lane 2 : Rat Brain lysates
Lane 3 : K562 treated with Lambda Protein Phosphatase for 30min whole cell
Lane 4 : K562 whole cell lysates
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 51 kDa

Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded human spleen tissue stained for PKA R2 (Phospho-S99）using 13379 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for PKA R2 (Phospho-S99）using 13379 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse heart tissue stained for PKA R2 (Phospho-S99) using 13379 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence PKA R2 (Phospho-S99) antibody (13379)

ICC/IF staining of PKA R2 (Phospho-S99) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13379 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence PKA R2 (Phospho-S99) antibody (13379)

ICC/IF staining of PKA R2 (Phospho-S99) in MCF-7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13379 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

If you have published an article using product 13379, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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