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Location： Home > Phospho Antibodies > ATF2(Phospho-T71) Rabbit mAb

ATF2(Phospho-T71) Rabbit mAb#13380

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Product NameATF2(Phospho-T71) Rabbit mAb

Clone No.SC05-90

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2

ConjugateUnconjugated

Other NamesActivating transcription factor 2 antibody
Activating transcription factor 2 splice variant ATF2 var2 antibody
ATF 2 antibody
Atf-2 antibody
Atf2 antibody
ATF2 protein antibody
ATF2_HUMAN antibody
cAMP Response Element Binding Protein 2 antibody
cAMP response element binding protein CRE BP1 antibody
cAMP response element-binding protein CRE-BP1 antibody
cAMP responsive element binding protein 2, formerly antibody
cAMP-dependent transcription factor ATF-2 antibody
cAMP-responsive element-binding protein 2 antibody
CRE BP1 antibody
CRE-BP antibody
CREB 2 antibody
CREB-2 antibody
CREB2 antibody
CREBP1 antibody
Cyclic AMP dependent transcription factor ATF 2 antibody
Cyclic AMP-dependent transcription factor ATF-2 antibody
Cyclic AMP-responsive element-binding protein 2 antibody
D130078H02Rik antibody
D18875 antibody
HB 16 antibody
HB16 antibody
Histone acetyltransferase ATF2 antibody
MGC105211 antibody
MGC105222 antibody
MGC111558 antibody
MGC142504 antibody
mXBP antibody
MXBP protein antibody
Tg(Gzma-Klra1)7Wum antibody
TREB 7 antibody
TREB7 antibody

Accession NoSwiss-Prot#:Q07954

Uniprot P15336

Gene ID 4035;

Calculated MWPredicted band size: 70 kDa

Sdspage MWObserved band size: 70 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : ATF2(Phospho-T71) Rabbit mAb at 1/1k dilution

Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : NIH/3T3 treated with 250nM Anisomycin for 30 minutes whole cell lysates
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with 250nM Anisomycin for 30 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 9 seconds
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for ATF2(Phospho-T71) using 13380 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for ATF2(Phospho-T71) using 13380 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for ATF2(Phospho-T71) using 13380 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence ATF2(Phospho-T71) antibody (13380)

ICC/IF staining of ATF2(Phospho-T71) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13380 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1.

If you have published an article using product 13380, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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