Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > TrkB(Phospho-Y817) Rabbit mAb

TrkB(Phospho-Y817) Rabbit mAb#13384

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameTrkB(Phospho-Y817) Rabbit mAb

Clone No.SC0556

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Tyr817 of human TrkB.

ConjugateUnconjugated

Accession NoSwiss-Prot#:Q16620

Uniprot Q16620

Gene ID 4915;

Calculated MWPredicted band size: 145 kDa

Sdspage MWObserved band size: 140 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : TrkB(Phospho-Y817) Rabbit mAb at 1/1k dilution

Lane 1 : SH-SY5Y whole cell lysates
Lane 2 : Mouse brain tissue lysates
Lane 3 : Rat brain tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 145 kDa
Observed band size: 140 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human kidney tissue stained for TrkB (Phospho-Y817) using 13384 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for TrkB (Phospho-Y817) using 13384 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for TrkB (Phospho-Y817) using 13384 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence TrkB(Phospho-Y817) antibody (13384)

ICC/IF staining of TrkB(Phospho-Y817) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13384 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The cells were incubated 30 minutes at room temperature with phalloidin tritc .
Nuclei were counterstained with DAPI.
The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3.

If you have published an article using product 13384, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors