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Location： Home > Phospho Antibodies > Stat5(Phospho-Y694) Rabbit mAb

Stat5(Phospho-Y694) Rabbit mAb#13386

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Product NameStat5(Phospho-Y694) Rabbit mAb

Clone No.SC05-31

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Tyr694 of human Stat5.

ConjugateUnconjugated

Other NamesMGF antibody
Signal transducer and activator of transcription 5A antibody
Signal Transducer and Activator of Transcription 5B antibody
STA5A_HUMAN antibody
STAT 5A antibody
STAT 5B antibody
STAT5 antibody
STAT5A antibody
STAT5B antibody
Transcription factor STAT5A antibody
Transcription factor STAT5B antibody

Accession NoSwiss-Prot#:P42229

Uniprot P42229

Gene ID 6776;

Calculated MWPredicted band size: 90 kDa

Sdspage MWObserved band size: 90 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes: Stat5(Phospho-Y694) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100ng/mL IFN alpha for 60 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 90 kDa
Observed band size: 90 kDa

Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human breast tissue stained for Stat5 (Phospho-Y694) using 13386 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Stat5 (Phospho-Y694) using 13386 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Stat5 (Phospho-Y694) antibody (13386)

ICC/IF staining of Stat5 (Phospho-Y694) in PC-12 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13386 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines. IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively. Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a. Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level. Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response.

If you have published an article using product 13386, please notify us so that we can cite your literature.

et al,Characterization of the rat oncostatin M receptor complex which resembles the human, but differs from the murine cytokine receptor. In PLoS One on 2012 by Johannes Drechsler, Joachim Gr枚tzinger, et al..PMID: 22937020 , (2012),
PMID: 22937020

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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