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Location： Home > Phospho Antibodies > SMC1(Phospho-S957) Rabbit mAb

SMC1(Phospho-S957) Rabbit mAb#13387

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Product Detail

Product NameSMC1(Phospho-S957) Rabbit mAb

Clone No.SC65-03

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser957 of human SMC1.

ConjugateUnconjugated

Other NamesCDLS2 antibody
DKFZp686L19178 antibody
DXhXs423e antibody
DXS423E antibody
KIAA0178 antibody
MGC138332 antibody
OTTHUMP00000061876 antibody
RP6 29D12.1 antibody
SB1.8 antibody
Segregation of mitotic chromosomes 1 antibody
Segregation of mitotic chromosomes like 1 antibody
SMC 1 antibody
SMC protein 1B antibody
SMC-1-beta antibody
SMC-1B antibody
SMC1 antibody
SMC1A antibody
SMC1alpha antibody
SMC1alpha protein antibody
SMC1B antibody
SMC1B_HUMAN antibody
SMC1BETA antibody
SMC1beta protein antibody
SMC1L1 antibody
SMC1L2 antibody
SMCB antibody
Structural maintenance of chromosome 1 like 1 protein antibody
Structural maintenance of chromosome 1 like 2 protein antibody
Structural maintenance of chromosomes 1A antibody
Structural maintenance of chromosomes 1B antibody
Structural maintenance of chromosomes protein 1B antibody

Accession NoSwiss-Prot#:Q14683

Uniprot Q14683

Gene ID 8243;

Calculated MWPredicted band size: 143 kDa

Sdspage MWObserved band size: 160 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes: SMC1(Phospho-S957) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with UV for 15 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 143 kDa
Observed band size: 160 kDa

Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for SMC1(Phospho-S957) using 13387 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human bladder cancer tissue stained for SMC1(Phospho-S957) using 13387 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence SMC1(Phospho-S957) antibody (13387)

ICC/IF staining of SMC1(Phospho-S957) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13387 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology.

If you have published an article using product 13387, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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