Product Detail
Product NameHsp27(Phospho-S82) Rabbit mAb
Clone No.SN06-73
Host SpeciesRabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB
Species ReactivityHu, Ms, Rt
Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser82 of human Hsp27.
ConjugateUnconjugated
Other NamesHeat shock 27kDa protein antibody
28 kDa heat shock protein antibody
CMT2F antibody
DKFZp586P1322 antibody
epididymis secretory protein Li 102 antibody
Estrogen regulated 24 kDa protein antibody
Estrogen-regulated 24 kDa protein antibody
Heat shock 25kDa protein 1 antibody
Heat shock 27 kDa protein antibody
Heat shock 27kD protein 1 antibody
Heat shock 27kDa protein 1 antibody
Heat shock 28kDa protein 1 antibody
Heat Shock Protein 27 antibody
Heat shock protein beta 1 antibody
Heat shock protein beta-1 antibody
heat shock protein family B (small) member 1 antibody
HEL-S-102 antibody
HMN2B antibody
HS.76067 antibody
Hsp 25 antibody
HSP 27 antibody
Hsp 28 antibody
Hsp B1 antibody
Hsp25 antibody
HSP27 antibody
Hsp28 antibody
HspB1 antibody
HSPB1_HUMAN antibody
SRP27 antibody
Stress responsive protein 27 antibody
Stress-responsive protein 27 antibody
Accession NoSwiss-Prot#:P04792
Uniprot
P04792
Gene ID
3315;
Calculated MWPredicted band size: 23 kDa
Sdspage MWObserved band size: 27 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2000
All lanes : Hsp27(Phospho-S82) Rabbit mAb at 1/1k dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Rat lung lysates
Lane 3 : Mouse lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 7 seconds
HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.
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